Sepia chirotrema is a species of cuttlefish native to the southern Indo-Pacific, specifically from Investigator Strait, southern Australia (35°25′S 137°22′E / 35.417°S 137.367°E / -35.417; 137.367) toDirk Hartog Island, western Australia (25°45′S 113°03′E / 25.750°S 113.050°E / -25.750; 113.050). It lives at a depth of between 120 and 210 m.
Sepia chirotrema grows to a mantle length of approximately 200 mm.
The type specimen was collected in the Investigator Strait area (35°25′S 137°22′E / 35.417°S 137.367°E / -35.417; 137.367), south of Kangaroo Island (35°50′S 137°15′E / 35.833°S 137.250°E / -35.833; 137.250). It was deposited at the Australian Museum in Sydney but no longer exists.