Hyloxalus maquipucuna is a species of frog in the family Dendrobatidae. It is endemic to Ecuador where it is only known from its type locality in the Maquipucuna reserve, in the Pichincha Province. Its name is a reference to this reserve.
Males measure 21 mm (0.83 in) and females 24 mm (0.94 in) in snout–vent length (based on 1 male and 2 females, the type series). It has moderately robust body. It is black dorsally and laterally, with a bright yellow dorsolateral stripe that extend to eye. Legs are orange and venter is yellow. Skin of dorsum is smooth (except slightly pustular in pelvic region).
