Anthidium florentinum, one of several European wool carder bees, is a territorial species of bee in the family Megachilidae, the leaf-cutter, carder, or mason bees.
The species is a medium to large bee. Both sexes have a black abdomen bearing two yellow bands on each tergite. The male can be distinguished by the grey and or whitish hairs on the sides of the abdomen. Females are smaller than males and have pale yellowish pollen brushes on the underside of the abdomen. The nest is made in a crevice or the abandoned nest of another insect and is lined with chewed plant fibres.
